To measure and quantify antibodies, antigens, hormones, and proteins in biological samples, researchers often turn to the enzyme-linked immunosorbent assay (ELISA). ELISA is a plate-based immunoassay technique that immobilizes the target antigen in a microplate, often 96-well or 384-well, and complexes it with an antibody linked to a reporter enzyme. Upon incubation with an appropriate substrate, detection of the reporter enzyme can occur for a measurable result. We recommend reading this article for more insight on the ELISA technique.
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