GSDMD ELISA kit (Human) (OKCD01821)

• Product Number: OKCD01821
• Name: GSDMD ELISA kit (Human) (OKCD01821)
• Protein Size (# AA): 484 amino acids
• NCBI Gene Id: 79792
• Gene Full Name: gasdermin D
• Description:
  View product citations for GSDMD ELISA kit (Human) on CiteAb
• Alias Symbols: DF5L;DFNA5L;FKSG10;gasdermin domain containing 1;gasdermin domain-containing protein 1;gasdermin-D;GSDMDC1.
• Description of Target: Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gasdermin D (GSDMD). No significant cross-reactivity or interference between Gasdermin D (GSDMD) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Sensitivity: < 0.116 ng/mL
• Uniprot ID: P57764
• Protein Name: Gasdermin-D
• Protein Accession #: NP_001159709.1
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Nucleotide Accession #: NM_001166237.1
• ELISA Kit Component:

  Component	Amount
  Anti-GSDMD Microplate	96 Wells (12 x 8 Well Strips)
  GSDMD Lyophilized Standard	2 x 20 ng
  100X Biotinylated GSDMD Detector Antibody	120 uL
  Avidin/HRP Conjugate	120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  TMB Substrate	1 x 9 mL
  Stop Solution	1 x 6 mL

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gasdermin D (GSDMD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gasdermin D (GSDMD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gasdermin D (GSDMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gasdermin D (GSDMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gasdermin D (GSDMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Gasdermin D (GSDMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Detection Method: Colorimetric
• COA Datasheet: ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Gene Symbol: GSDMD
• Bulk Note: Please contact customer service when ordering 10+ kits.
• Predicted Species Reactivity: Homo sapiens|Human
• Application: ELISA-Sandwich
• Image 1: GSDMD ELISA Standard Curve
  
  Human GSDMD ELISA Kit Standard Curve