GSDMD Antibody (OACA03071)

• Product Number: OACA03071
• Name: GSDMD Antibody (OACA03071)
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 79792
• Host: Rabbit
• Clonality: Polyclonal
• Purity: >95%
• Concentration: Varies by lot. See vial for exact concentration.
• Gene Full Name: gasdermin D
• Alias Symbols: DF5L;DFNA5L;FKSG10;gasdermin domain containing 1;gasdermin domain-containing protein 1;gasdermin-D;GSDMDC1.
• Product Format: Liquid
• Description of Target: Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
• Reconstitution and Storage: -20°C or -80°C
• Application Info: WB: 1:1000~2000
• Immunogen: Recombinant Human Gasdermin-D protein (1-256AA)
• Uniprot ID: P57764
• Protein Name: Gasdermin-D
• Protein Accession #: NP_001159709
• Purification: Protein G Purified
• Nucleotide Accession #: NM_001166237
• Gene Symbol: GSDMD
• Predicted Species Reactivity: Human
• Application: ELISA, WB
• Image 1: Jurkat whole cell lysate, Hela whole cell lysate, A431 whole cell lysate, U251 whole cell lysate
  
  Western blot
  All lanes: GSDMD antibody at 4ug/ml
  Lane 1: Jurkat whole cell lysate
  Lane 2: Hela whole cell lysate
  Lane 3: A431 whole cell lysate
  Lane 4: U251 whole cell lysate
  Secondary
  Goat polyclonal to Rabbit IgG at 1/1,0000 dilution
  Predicted band size: 53 kDa
  Observed band size: 53 kDa