Denatured ULK1 Antibody (OAAB05707)

• Product Number: OAAB05707
• Name: Denatured ULK1 Antibody (OAAB05707)
• Molecular Weight: 112631 Da
• Isotype: Rabbit Ig
• NCBI Gene Id: 8408
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Alias Symbols: ATG1, ATG1A, UNC51, hATG1, Unc51.1
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: Function: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
  
• Additional Information: Cellular Location: Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome
  Tissue Location: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: Denatured ATG1 Antibody flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Immunogen: This denatured ATG1 antibody is generated from rabbits immunized with a denatured human ATG1 recombinant protein.
• Uniprot ID: O75385
• Protein Name: Serine/threonine-protein kinase ULK1
• Protein Accession #: NP_003556.1
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Gene Symbol: ULK1
• Predicted Species Reactivity: Human, Mouse
• Application: FC, IHC-P, WB
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Image 1: mouse cerebellum
  
  Western blot analysis of Denatured ATG1 in mouse cerebellum tissue lysates (35ug/lane). ATG1 (arrow) was detected using the purified Pab.
• Image 2: Human Skeletal Muscle
  
  Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Denatured ATG1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
• Image 3: k562
  
  Denatured ATG1 Antibody flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.