ULK1 Antibody - N-terminal region (ARP54261_P050)

• Product Number: ARP54261_P050
• Name: ULK1 Antibody - N-terminal region (ARP54261_P050)
• Protein Size (# AA): 1050 amino acids
• Molecular Weight: 112 kDa
• NCBI Gene Id: 8408
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: unc-51-like kinase 1 (C. elegans)
• Alias Symbols: ATG1, ATG1A, UNC51, hATG1, Unc51.1
• Peptide Sequence: Synthetic peptide located within the following region: PEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: ULK1 is a serine/threonine-protein kinase involved in autophagy in response to starvation. It acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. It is activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. It may phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences and plays a role early in neuronal differentiation and is required for granule cell axon formation.
• Protein Interactions: SESN2; SQSTM1; ATG16L1; EXOC8; EXOC2; BECN1; RALB; AMBRA1; ULK1; UBC; TRAF6; MBP; ATG13; MAP1LC3C; MAP1LC3A; MAP1LC3B; GABARAPL1; GABARAPL2; GABARAP; MLST8; RPTOR; MTOR; PPP1CA; ATG4B; CDC37; G3BP1; RB1CC1; USP10; PRKAG1; PRKAB2; PRKAA2; PRKAA1; SYNGAP1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-ULK1 (ARP54261_P050) antibody is Catalog # AAP54261
• Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human ULK1
• Uniprot ID: O75385
• Protein Name: Serine/threonine-protein kinase ULK1
• Protein Accession #: NP_003556
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003565
• Tested Species Reactivity: Human
• Gene Symbol: ULK1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Thymus Tumor
  
  Host: Rabbit
  Target Name: ULK1
  Sample Type: Thymus Tumor lysates
  Antibody Dilution: 1.0ug/ml