SPP1 Antibody - N-terminal region (ARP36676_P050)

Data Sheet
 
Product Number ARP36676_P050
Product Page staging.avivasysbio.com/spp1-antibody-n-terminal-region-arp36676-p050.html
Name SPP1 Antibody - N-terminal region (ARP36676_P050)
Protein Size (# AA) 300 amino acids
Molecular Weight 34kDa
NCBI Gene Id 6696
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Secreted phosphoprotein 1
Alias Symbols OPN, BNSP, BSPI, ETA-1
Peptide Sequence Synthetic peptide located within the following region: MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQ
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Emani,S., (2008) Surgery 143 (6), 803-812
Description of Target The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.
Protein Interactions SGTA; MEOX2; PARP1; BAG6; PEPD; APOA1; UBQLN4; CISD2; TUBB; CC2D1B; COMMD1; RIMS4; C2orf50; CMTM3; DSEL; ZNF804A; USP42; TMEM168; LRRC4; GRIPAP1; TMEM30A; AMOTL2; C1RL; DNTTIP2; KCNIP1; STK39; EIF3K; ZNF638; AFF4; CACNG4; SLC39A6; PLD3; SNAPIN; NCSTN; CEP
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-SPP1 (ARP36676_P050) antibody
Blocking Peptide For anti-SPP1 (ARP36676_P050) antibody is Catalog # AAP36676 (Previous Catalog # AAPP08103)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human SPP1
Uniprot ID B2RDA1
Protein Name Osteopontin
Protein Accession # NP_000573
Purification Affinity Purified
Nucleotide Accession # NM_000582
Tested Species Reactivity Human
Gene Symbol SPP1
Predicted Species Reactivity Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Dog: 93%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 91%; Rabbit: 93%; Rat: 92%
Image 1
Human THP-1
WB Suggested Anti-SPP1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: THP-1 cell lysate
Image 2
Mouse Pancreas, Mouse Heart
Host: Rabbit
Target: SPP1
Positive control (+): Mouse Pancreas (M-PA)
Negative control (-): Mouse Heart (M-HE)
Antibody concentration: 1ug/ml
 

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